General Cell Based Assays
Abcam Urea Assay Kit II
Urea Assay Kit II
The product is subject to the following: Abcam Restricted Use Statement
Abcam Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)
Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Lysosomal Intracellular Activity Assay Kit
Lysosomal Intracellular Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
American Research Products Inc Bovine LPO ELISA Kit
This Bovine Lactoperoxidase (LPO) ELISA Kit is a precoated Sandwich ELISA assay designed to detect and quantify the target analyte in milk tears and other biological fluids This product is for Research Use Only
Cell Signaling Technology Active Ras Detection Kit, 30 Immunoprecipitations
The Active Ras Detection Kit provides the necessary reagents for measuring the activation of Ras GTPase in cells. The assay utilizes a GST-Raf1-RBD fusion protein to specifically bind the activated, GTP-bound form of Ras, which is subsequently immunoprecipitated using glutathione resin. Ras activation levels are then quantified via western blot analysis using a Ras mouse monoclonal antibody.
- Enables detection of endogenous levels of GTP-bound (active) Ras
- Includes all reagents for immunoprecipitation of Ras-GTP
- Validated for use with human, mouse, and rat samples
- Supports functional analysis of Ras signaling
- Includes controls for positive and negative signal verification
Montana Molecular Green Cilia-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O.
Meso Scale Discovery RPLEX HUM CK Beta 81/CCL23 ASY
The Human CK-beta-8-1/CCL23 DuoSet ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CK-beta-8-1/CCL23 in cell culture supernatants serum plasma and other biological fluids This immunoassay contains recombinant human CK-beta-8-1/CCL23 and antibodies raised against the recombinant factor allowing for the detection of natural and recombinant human CK-beta-8-1/CCL23 The ELISA has a sensitivity of 7 8 pg/mL and a detection range of 15 6-1000 pg/mL This product can be used to measure CK-beta-8-1/CCL23 levels for the study of chemokine biology and inflammation
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that measures mouse macrophage inflammatory protein 1-alpha (MIP-1alpha) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluent The assay takes approximately 3 5 hours to run and 10 L of sample is required The kit can test up to 80 samples in duplicate
Meso Scale Discovery U-PLEX Rat Leptin ASY
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and controls The assay requires a small sample volume and provides results in as little as 3 5 hours With minimal hands-on time this kit allows for the rapid accurate quantification of rat leptin
Meso Scale Discovery R-PLEX Human IL-23R ASY
The Meso Scale Discovery R-PLEX Human IL-23R Assay is a multiplex immunoassay for measuring IL-23R in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on patterned arrays for high sensitivity and a wide dynamic range The kit includes a plate with capture antibodies detection antibodies labeled with an electrochemiluminescent compound and calibrator and control samples for standard curve generation and assay validation It quantifies IL-23R with high precision and reproducibility suitable for 96 tests on MSD platforms
Lonza Walkersville PPiLight Inorganic Pyrophosphate Assay, 500 Test
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. Benefits:Fast - Measure enzyme activity via pyrophosphate consumption in 1 hourSimple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substratesWide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µMVersatile - Scalable to 96-, 384-, 1586-well formatsApplications:Measure activity of:PhosphotransferasesPyrophosphotasesCyclasesLigasesHydrolasesDNA Polymerases
Meso Scale Discovery R-PLEX Human Trk-A total ASY
The R-PLEX Human TrkA Total Assay Kit from Meso Scale Discovery measures total TrkA in human cell and tissue lysates This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array plate technology for high sensitivity and minimal matrix interference The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay accurately quantifies total TrkA levels
Eurofins Discoverx PathHunter eXpress mCXCR4 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0481E3MCP5M)
The PathHunter mCXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR4 and EA-tagged β-Arrestin-2. Activation of mCXCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0400E2CP2L)
The PathHunter OPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of OPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRD1 and EA-tagged β-Arrestin-2. Activation of OPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL7R/CRLF2 Dimerization Assay, 10pl Kit. (93-1019E13CP25L)
The PathHunter IL7R/CRLF2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.